Neuraminidase B (NanB)
Product summary
Neuraminidase B (NanB) from Streptococcus pneumoniae selectively cleaves terminal α2-3-linked sialic acids from glycoconjugates and can generate 2,7-anhydro-Neu5Ac through its transglycosidase-like activity. It is used for linkage-specific glycoprotein/glycan analysis, controlled desialylation in bioprocessing, production of analytical sialic-acid standards, and enzymatic synthesis or modification of glycoconjugates.
Product Description
Neuraminidase B (NanB) from Streptococcus pneumoniae is a sialidase enzyme that hydrolyzes terminal sialic acid residues from glycoconjugates, similar to NanA, but with distinct substrate preferences. NanB exhibits higher specificity toward α2-3-linked sialic acids and tends to act more efficiently on oligosaccharides and glycoproteins containing these linkages. Unlike NanA, which shows broad desialylation activity, NanB often functions in a more selective manner and can produce 2,7-anhydro-Neu5Ac as a reaction product rather than free sialic acid, reflecting its transglycosidase-like mechanism. Its selective activity makes it valuable for analytical and structural studies where controlled or linkage-specific desialylation is required (Ref).
Additonal Information
| Product Name | Sialidase B |
|---|---|
| Synonyms | Neuraminidase B, Nan. |
| Source (Organism/Expression) | Streptococcus pneumoniae, expressed in E. coli |
| UniProt | Q54727 |
| Molecular Weight | 74 kDa (appears 70–100 kDa on SDS-PAGE) |
| Isoform / Subunit Structure | Amino acids 40–697 with C-terminal 6×His tag |
| Storage Buffer | 20 mM Tris-HCl, 100 mM NaCl, 10 % vol/vol glycerol, pH 7.5 |
| Concentration | 6.9 U/μL |
| Storage Conditions | −80 °C |
| Recommended Applications | glycoprotein and glycan structural analysis, removal of sialic acids in biopharmaceutical processing, production of analytical standards for sialic acid quantification, and enzymatic synthesis or modification of glycoconjugates. |
| Catalogue Number | – |
| Purity | >95% by SDS-PAGE (see Figure 1) |
| Enzyme Activity Definition | 1 unit (U) of enzyme activity is the amount of enzyme that catalyzes the conversion of 1 µmol of 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid (4-MUNANA) per minute under specified assay conditions specified as follows: -Buffer: 66mM MES, 8mM CaCl2, 25 mM Na2CO3, pH 6.5. |
