Key Features
Universal Kinase Compatibility
Supports diverse kinase classes—serine/threonine, tyrosine, lipid, and metabolic kinases—independent of substrate identity.
Continuous Real-Time Readout
NADPH fluorescence is monitored continuously, enabling precise measurement of phosphorylation rates, catalytic efficiency, and substrate preference.
Robust Coupled-Enzyme System
An optimized combination of sugar kinases and glucose-6-phosphate dehydrogenase converts ADP release into a strong, highly sensitive fluorescent signal.
Standardized Platform for Inhibitor Discovery
Fixed reaction chemistry ensures high reproducibility, making UKC 1.0 suitable for high-throughput inhibitor screening, IC₅₀ determination, mode-of-inhibition studies, and comparative profiling of inhibitor or substrate libraries.
Assay Principle
Universal Kinase Compatibility
Supports diverse kinase classes—serine/threonine, tyrosine, lipid, and metabolic kinases—independent of substrate identity.
Continuous Real-Time Readout
NADPH fluorescence is monitored continuously, enabling precise measurement of phosphorylation rates, catalytic efficiency, and substrate preference.
Robust Coupled-Enzyme System
An optimized combination of sugar kinases and glucose-6-phosphate dehydrogenase converts ADP release into a strong, highly sensitive fluorescent signal.
Standardized Platform for Inhibitor Discovery
Fixed reaction chemistry ensures high reproducibility, making UKC 1.0 suitable for high-throughput inhibitor screening, IC₅₀ determination, mode-of-inhibition studies, and comparative profiling of inhibitor or substrate libraries.
Kit Components
Enzyme Cocktail
- Sugar kinases optimized to efficiently convert ADP released from kinase reactions
- Glucose-6-phosphate dehydrogenase tuned for continuous NADP⁺ reduction and stable fluorescent output
Assay Buffer
- Formulated for broad kinase compatibility, including required intermediate substrates
Optional Add-Ons (upon request)
- Positive control kinase
- Positive control peptide or protein substrate
User Guide
- Detailed protocol for kinetic assays, substrate profiling, and high-throughput inhibitor screening (HTS)
