Key Features
Universal GT Compatibility
Works with a wide range of glycosyltransferases, independent of donor or acceptor substrate, enabling broad applicability across GT families.
Continuous Real-Time Readout
NADPH fluorescence is monitored continuously, providing high-resolution kinetic data for determining catalytic efficiency, donor preference, and reaction dynamics.
Robust Coupled-Enzyme System
The optimized coupling cocktail converts nucleotide release (UDP, GDP, CMP) into a clean, amplified fluorescent signal, ensuring sensitivity and reproducibility across assays.
Standardized Platform for Inhibitor Discovery
Fixed, validated reaction chemistry eliminates assay-to-assay variability, making UGC 2.0 ideal for high-throughput screening, IC₅₀ determination, mode-of-inhibition studies, and comparative profiling of inhibitor libraries.
Assay Principle
Universal GT Compatibility
Works with a wide range of glycosyltransferases, independent of donor or acceptor substrate, enabling broad applicability across GT families.
Continuous Real-Time Readout
NADPH fluorescence is monitored continuously, providing high-resolution kinetic data for determining catalytic efficiency, donor preference, and reaction dynamics.
Robust Coupled-Enzyme System
The optimized coupling cocktail converts nucleotide release (UDP, GDP, CMP) into a clean, amplified fluorescent signal, ensuring sensitivity and reproducibility across assays.
Standardized Platform for Inhibitor Discovery
Fixed, validated reaction chemistry eliminates assay-to-assay variability, making UGC 2.0 ideal for high-throughput screening, IC₅₀ determination, mode-of-inhibition studies, and comparative profiling of inhibitor libraries.
Kit Components
Enzyme Cocktail
- Nucleotide kinases tailored for sensitive detection of CMP, UDP, and GDP release
- Sugar kinases and dehydrogenase optimized to continuously couple kinase products to NADP⁺ reduction
Assay Buffer
- Formulated for broad glycosyltransferase compatibility, including required intermediate substrates
Optional Add-Ons (upon request)
- Positive control glycosyltransferase
- Positive control donor/acceptor substrate
User Guide
- Detailed protocol for kinetic assays, substrate profiling, and high-throughput screening (HTS)
