Neuraminidase A (NanA)
Product summary
Neuraminidase A (NanA) from Streptococcus pneumoniae cleaves terminal α2-3 and α2-6 sialic acids from glycoconjugates, exposing underlying sugars. It is used for glycoprotein/glycan analysis, biopharmaceutical desialylation, and enzymatic synthesis or modification of glycoconjugates.
Product Description
Neuraminidase A (NanA) from Streptococcus pneumoniae is an enzyme that catalyzes the hydrolysis of terminal sialic acid residues from glycoproteins, glycolipids, and oligosaccharides. It acts specifically on α-ketosidic linkages of sialic acids, releasing free sialic acid and exposing underlying sugar residues. NanA efficiently cleaves both α2-3 and α2-6 linkages, making it useful for broad desialylation applications.(Ref).
Additonal Information
| Product Name | Sialidase A |
|---|---|
| Synonyms | Neuraminidase A, NanA. |
| Source (Organism/Expression) | Streptococcus pneumoniae, expressed in E. coli |
| UniProt | P62575 |
| Molecular Weight | 86 kDa (appears 70–100 kDa on SDS-PAGE) |
| Isoform / Subunit Structure | Amino acids 122–883 with C-terminal 6×His tag |
| Storage Buffer | 20 mM Tris-HCl, 100 mM NaCl, 10 % vol/vol glycerol, pH 7.5 |
| Concentration | 7.3 U/μL |
| Storage Conditions | −80 °C |
| Recommended Applications | glycoprotein and glycan structural analysis, removal of sialic acids in biopharmaceutical processing, production of analytical standards for sialic acid quantification, and enzymatic synthesis or modification of glycoconjugates. |
| Catalogue Number | – |
| Purity | >95% by SDS-PAGE (see Figure 1) |
| Enzyme Activity Definition | 1 unit (U) of enzyme activity is the amount of enzyme that catalyzes the conversion of 1 µmol of 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid (4-MUNANA) per minute under specified assay conditions specified as follows: -Buffer: 66mM MES, 8mM CaCl2, 25 mM Na2CO3, pH 6.5. |
